il 13 duoset elisa  (R&D Systems)

Journal: Cells

Article Title: Differential Responsiveness of Human Skin Mast Cells to SCF and IL-33: Reduced Reactivity to SCF but Not to IL-33 in the Post-Mitotic Phase

doi: 10.3390/cells15050398

Figure Lengend Snippet: IL-33 and SCF differentially modulate cytokine production in skin MCs. ( a ) mRNA levels of CCL1 , TNF-α , IL-13 , and LIF were assessed by RT-qPCR following stimulation with IL-33 (green) or SCF (purple) for 90 min. Expression was normalized to three housekeeping genes ( ACTB , GAPDH , and PPIB ) and is presented relative to the unstimulated control. ( b ) Protein concentrations of CCL1, TNF-α, IL-13, and LIF were determined by ELISA in culture supernatants after stimulation. The data are presented as individual dots with mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns: not significant. The figures were created using GraphPad Prism 10.4.2 (GraphPad Software, La Jolla, CA, USA, accessed on 23 February 2026).

Article Snippet: ELISA assays were performed to quantify cytokine levels using the following kits, according to the manufacturers’ instructions: Human LIF ELISA Kit (Cat# BMS242, Thermo Fisher Scientific, Berlin, Germany), Human IL-13 DuoSet ELISA (Cat# DY213, R&D Systems, Wiesbaden, Germany), Human CCL1/I-309 DuoSet ELISA (Cat# DY272, R&D Systems), and Human TNF-α ELISA Kit (Cat# 88-7346-22, Thermo Fisher Scientific).

Techniques: Quantitative RT-PCR, Expressing, Control, Enzyme-linked Immunosorbent Assay, Software

Journal: Cells

Article Title: Differential Responsiveness of Human Skin Mast Cells to SCF and IL-33: Reduced Reactivity to SCF but Not to IL-33 in the Post-Mitotic Phase

doi: 10.3390/cells15050398

Figure Lengend Snippet: IL-33-induced cytokine responses tend to be more pronounced in post-mitotic cells, while SCF responsiveness remains broadly comparable between lifecycle phases. ( a ) mRNA levels of CCL1 , TNF-α , IL-13 and LIF were assessed by RT-qPCR in proliferative and post-mitotic MCs following stimulation with IL-33 (green) or SCF (purple) for 90 min. Expression was normalized as described in Methods and is presented relative to the unstimulated control. ( b ) Protein concentrations of CCL1, TNF-α, IL-13, and LIF were determined by ELISA in culture supernatants from proliferative and post-mitotic MCs. The data are presented as individual dots with mean ± SEM. * p < 0.05; **** p < 0.0001. pm indicates post-mitotic MCs; proli indicates proliferative MCs. The figures were created using GraphPad Prism 10.4.2 (GraphPad Software, La Jolla, CA, USA, accessed on 23 February 2026).

Article Snippet: ELISA assays were performed to quantify cytokine levels using the following kits, according to the manufacturers’ instructions: Human LIF ELISA Kit (Cat# BMS242, Thermo Fisher Scientific, Berlin, Germany), Human IL-13 DuoSet ELISA (Cat# DY213, R&D Systems, Wiesbaden, Germany), Human CCL1/I-309 DuoSet ELISA (Cat# DY272, R&D Systems), and Human TNF-α ELISA Kit (Cat# 88-7346-22, Thermo Fisher Scientific).

Techniques: Quantitative RT-PCR, Expressing, Control, Enzyme-linked Immunosorbent Assay, Software